1. Tune the 1st (high e) and 6th (low E) strings
Tune the 6th (low E) string using a tuner or by ear, referencing a well-tuned piano/keyboard.
Play the harmonic at the 5th fret of the 6th (low E) string. This is your reference tone.
As it sounds, adjust the 1st (high e) string until the open string is in pure unison with your reference tone.
You now have the 1st (high e) and 6th (low E) strings in tempered tuning.
2. Tune the 4th (D) string
Play a harmonic at the 12th fret of the, now in tune, 6th (low E) string. This is your reference tone.
As it sounds, play an E note on the 2nd fret of the 4th (D) string and adjust the string until that E is in perfect unison with your reference tone.
You now have the 1st (high e), 4th (D) and 6th (low E) strings in tempered tuning.
3. Tune the 2nd (b) string
As above, play a harmonic at the 12th fret of the 4th (D) string. This is your reference tone.
As it sounds, play a D on the 3rd fret of the 2nd (B) string and adjust the string until that D is in perfect unison with your reference tone.
You now have the 1st (high e), 2nd (B), 4th (D) and 6th (low E) strings in tempered tune.
4. Tune the 3rd (G) string
Play a harmonic tone at the 12th fret of the, now in tune, 4th (D) string. This is your reference tone.
As it sounds, play a D on the 7th fret of the 3rd (G) string and adjust the string until that D is in perfect unison with your reference tone.
Double check: play a harmonic tone at the 12th fret of the 3rd (G) string against a G played on the 3rd fret of the 1st (high e) string. The two notes should be in perfect unison.
You now have the 1st (high e), 2nd (B), 3rd (G), 4th (D) and 6th (low E) strings in tempered tune.
5. Tune the 5th (A) string
Play an A note on 2nd fret of the, now in tune, 3rd (G) string. This is your reference tone.
As it sounds, play a harmonic at the 12th fret of the 5th (A) string, and adjust the string until that A is in perfect unison with your A at the 2nd fret of the 3rd (G) string.
Your guitar is now tuned to equal temperamen